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Opsin 3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NLS2134

Novus Biologicals, part of Bio-Techne
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NLS2134

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Monkey (94%), Primate (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from C-terminus of human Opsin 3.

Epitope

C-Terminus

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21700660). Predicted cross-reactivity based on sequence identity: Gibbon (94%), Marmoset (94%). Rat reactivity reported from a verified customer review.

Localization

Cell Membrane

Specificity

Human Opsin 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Opsin 3 Antibody - BSA Free

Immunohistochemistry Free-Floating: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry Free-Floating: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry Free-Floating: Opsin 3 Antibody [NLS2134] - Rat brain samples (cerebral cortex). Rat brain 40um free floating sections were incubated with Opsin 3 Antibody (NLS2134) at 1:250 overnight at 4 degrees C. The following day, sections were incubated with anti-rabbit Alexa Fluor Plus 488 1:500 and DAPI. Opsin 3 Antibody (NLS2134; 1:250) in green. Nuclei stained with DAPI (blue). IHC image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Analysis of anti-Encephalopsin / OPN3 antibody with brain, neurons and glia at 5 ug/mL.
Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Analysis of anti-Encephalopsin / OPN3 antibody with human skin, melanoma.

Applications for Opsin 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 - 8 ug/mL
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 21700660). This Opsin 3 Antibody is validated for IHC-FrFl from a verified customer review..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NLS2134 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Opsin 3

Encephalopsin, a member of the Opsin subfamily of GPCRs, is similar in sequence to vertebrate retinal and pineal opsins and may affect photoreception in the brain. Encephalopsin expression has been documented in various brain tissues, including the preoptic area and paraventricular nucleus of the hypothalamus, cerebral cortex, cerebellar Purkinje neurons, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord. ESTs have been isolated from human blood, breast, cervix, colon, germ cell, head/neck, kidney, lung, pancreas, placenta, prostate, skin, testis, and uterus cancer libraries, as well as from normal human blood, brain, eye, heart, kidney, liver, liver/spleen, ovary, placenta, prostate, skin, testis, and B-cell/lung/testis libraries.

Alternate Names

ECPN, ECPNopsin-3, encephalopsin, ERO, NMO-1, opsin 3, opsin 3 (encephalopsin, panopsin), Panopsin

Entrez Gene IDs

23596 (Human)

Gene Symbol

OPN3

Additional Opsin 3 Products

Product Documents for Opsin 3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Opsin 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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