Optimedin Antibody (3A2)

Novus Biologicals, part of Bio-Techne | Catalog # H00118427-M06

Novus Biologicals, part of Bio-Techne
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H00118427-M06
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Optimedin Antibodies »

Product Specifications

Immunogen

OLFM3 (NP_477518, 108 a.a. ~ 206 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FRQIEDDRKTLMTKHFQELKEKMDELLPLIPVLEQYKTDAKLITQFKEEIRNLSAVLTGIQEEIGAYDYEELHQRVLSLETRLRDCMKKLTCGKLMKIT

Specificity

OLFM3 - olfactomedin 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Optimedin Antibody (3A2)

Western Blot: Optimedin Antibody (3A2) [H00118427-M06]

Western Blot: Optimedin Antibody (3A2) [H00118427-M06]

Western Blot: Optimedin Antibody (3A2) [H00118427-M06] - Analysis of OLFM3 expression in Raw 264.7 (Cat # L024V1).
Western Blot: Optimedin Antibody (3A2) [H00118427-M06]

Western Blot: Optimedin Antibody (3A2) [H00118427-M06]

Western Blot: Optimedin Antibody (3A2) [H00118427-M06] - Analysis of OLFM3 expression in PC-12 (Cat # L012V1).
Western Blot: Optimedin Antibody (3A2) [H00118427-M06]

Western Blot: Optimedin Antibody (3A2) [H00118427-M06]

Western Blot: Optimedin Antibody (3A2) [H00118427-M06] - Analysis of OLFM3 expression in NIH/3T3 (Cat # L018V1).
View 1 more images

Applications for Optimedin Antibody (3A2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Optimedin

Optimedin, also known as olfactomedin 3 (OLFM3) and Noelin-3, is a protein expressed in the eyes, brain, and lungs. The protein is known to stimulate the formation of cell adherent and cell-cell tight junctions. Additionally, its expression modulates cell migration and the organization of the cytoskeleton. In one study, cells expressing high levels of optimedin showed higher growth rates and stronger adhesion to the collagen extracellular matrix as compared with control cells. Optimedin has also been identified as a candidate gene for glaucoma.

Alternate Names

NOE3OPTIMEDIN, noelin 3, NOELIN3, noelin-3, NOELIN3_V1, NOELIN3_V2, NOELIN3_V3, NOELIN3_V4, NOELIN3_V5, NOELIN3_V6, olfactomedin 3, olfactomedin related ER localized protein 3, Olfactomedin-3, Optimedin

Entrez Gene IDs

118427 (Human)

Gene Symbol

OLFM3

OMIM

607567 (Human)

UniProt

NP_477518 (Human)

Additional Optimedin Products

Product Documents for Optimedin Antibody (3A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Optimedin Antibody (3A2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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