Optineurin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00298

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Optineurin Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Optineurin (Accession#: NP_068815.2; Met1-Ile577)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Optineurin Antibody

Western Blot: Optineurin Antibody [NBP3-00298]

Western Blot: Optineurin Antibody [NBP3-00298]

Western Blot: Optineurin Antibody [NBP3-00298] - Anti-Optineurin rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: Hela Whole Cell Lysate. Lane D: NCI-H1299 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 66 kDa. Observed band size: 75 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Optineurin Antibody [NBP3-00298]

Immunoprecipitation: Optineurin Antibody [NBP3-00298]

Immunoprecipitation: Optineurin Antibody [NBP3-00298] - Optineurin was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Optineurin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Optineurin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 66 kDa. Observed band size: 75 kDa

Applications for Optineurin Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Optineurin

Optineurin encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Alternate Names

ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, OPTN

Gene Symbol

OPTN

Additional Optineurin Products

Product Documents for Optineurin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Optineurin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.