ORC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83184

Novus Biologicals, part of Bio-Techne
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NBP1-83184
NBP1-83184-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ORC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YISGVPGTGKTATVHEVIRCLQQAAQANDVPPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAELLAKQFCTRGSPQETTVLLVDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ORC1 Antibody

Immunocytochemistry/ Immunofluorescence: ORC1 Antibody [NBP1-83184]

Immunocytochemistry/ Immunofluorescence: ORC1 Antibody [NBP1-83184]

Immunocytochemistry/Immunofluorescence: ORC1 Antibody [NBP1-83184] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ORC1 Antibody [NBP1-83184]

Immunohistochemistry-Paraffin: ORC1 Antibody [NBP1-83184]

Immunohistochemistry-Paraffin: ORC1 Antibody [NBP1-83184] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ORC1 Antibody [NBP1-83184]

Immunohistochemistry-Paraffin: ORC1 Antibody [NBP1-83184]

Immunohistochemistry-Paraffin: ORC1 Antibody [NBP1-83184] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
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Applications for ORC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ORC1

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. [provided by RefSeq]

Alternate Names

HS, HSORC1, ORC1Lorigin recognition complex subunit 1, origin recognition complex, subunit 1, origin recognition complex, subunit 1 (yeast homolog)-like, origin recognition complex, subunit 1 homolog, origin recognition complex, subunit 1 homolog (S. cerevisiae), origin recognition complex, subunit 1-like, origin recognition complex, subunit 1-like (yeast), PARC1origin recognition complex 1, Replication control protein 1

Entrez Gene IDs

4998 (Human)

Gene Symbol

ORC1

UniProt

Q13415 (Human)

Additional ORC1 Products

Product Documents for ORC1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ORC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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