Orexin B/Hypocretin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42905

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Human (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Orexin B/Hypocretin-2 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Orexin B/Hypocretin-2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Orexin B/Hypocretin-2 Antibody

Orexin B/Hypocretin-2 Antibody

Western Blot: Rabbit Polyclonal Orexin B/Hypocretin-2 Antibody [NBP2-42905] -

Western Blot: Rabbit Polyclonal Orexin B/Hypocretin-2 Antibody [NBP2-42905] - hypocretin (orexin) neuropeptide precursor antibody detects hypocretin (orexin) neuropeptide precursor protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with hypocretin (orexin) neuropeptide precursor antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Orexin B/Hypocretin-2 Antibody

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Orexin B/Hypocretin-2

Orexin A and orexin B are neuropeptide hormones derived from proteolytic processing of the same orexin precursor protein. Orexins are named for their orexigenic-inducing (orexis; Greek for appetite) activity. They are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. Orexins A and B are less than 50% amino acid identical. Orexin A is a ligand for two 7-transmembrane orexin receptors, OX1R and OX2R, while orexin B preferentially binds OX2R. Defects in the orexin precursor are a cause of narcolepsy in humans.

Alternate Names

Hcrt2, Hypocretin-2, Hypocretin2, PPORX, PPOX

Gene Symbol

HCRT

Additional Orexin B/Hypocretin-2 Products

Product Documents for Orexin B/Hypocretin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Orexin B/Hypocretin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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