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Orexin Receptor 1/2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93465

Novus Biologicals, part of Bio-Techne
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NB100-93465

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-YNFLSGKFREQFK corresponding to internal region according to NP_001517.2.

Specificity

This antibody is expected to recognise both the human proteins OX1R and OX2R.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Orexin Receptor 1/2 Antibody

Western Blot: Orexin Receptor 1/2 Antibody [NB100-93465]

Western Blot: Orexin Receptor 1/2 Antibody [NB100-93465]

Western Blot: Orexin Receptor 1/2 Antibody [NB100-93465] - Staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Orexin Receptor 1/2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human brain (frontal cortex), mouse brain and rat brain lysates (calculated MW of 47.5 kDa band according to NP_001516.1 and NP_001517.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Orexin Receptor 1/2

The protein encoded by the Orexin Receptor 1/2 gene is a G-protein coupled receptor involved in the regulation of feeding behavior. Theencoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-proteincoupled receptor that binds orexin A and orexin B. (provided by RefSeq)

Alternate Names

hypocretin (orexin) receptor 1, hypocretin receptor 1, Hypocretin receptor type 1, hypocretin receptor-1, orexin receptor 1, orexin receptor type 1, orexin receptor-1, OX1R, Ox-1-R, ox1-R

Entrez Gene IDs

3061 (Human); 230777 (Mouse); 25593 (Rat)

Gene Symbol

HCRTR1

UniProt

Additional Orexin Receptor 1/2 Products

Product Documents for Orexin Receptor 1/2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Orexin Receptor 1/2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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