OSBP1 Antibody (OTI1F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00935

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F2

Concentration

0.33 mg/ml

View all OSBP1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human OSBP (NP_002547) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

89.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OSBP1 Antibody (OTI1F2)

Western Blot: OSBP1 Antibody (OTI1F2) [NBP2-00935]

Western Blot: OSBP1 Antibody (OTI1F2) [NBP2-00935]

Western Blot: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.
Immunocytochemistry/Immunofluorescence: OSBP1 Antibody (1F2) [NBP2-00935] Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP1.
Immunohistochemistry-Paraffin: OSBP1 Antibody (OTI1F2) [NBP2-00935]

Immunohistochemistry-Paraffin: OSBP1 Antibody (OTI1F2) [NBP2-00935]

Immunohistochemistry-Paraffin: OSBP1 Antibody (OTI1F2) [NBP2-00935] - Staining of paraffinembedded Adenocarcinoma of Humanendometrium tissue using anti-OSBP mousemonoclonal antibody. (Heat-induced epitoperetrieval by 10mM citric buffer, pH6.0, 100 degrees C for10min, TA503140)
View 12 more images

Applications for OSBP1 Antibody (OTI1F2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.33 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OSBP1

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq].

Alternate Names

OSBP1oxysterol-binding protein 1, oxysterol binding protein

Entrez Gene IDs

5007 (Human)

Gene Symbol

OSBP

Additional OSBP1 Products

Product Documents for OSBP1 Antibody (OTI1F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OSBP1 Antibody (OTI1F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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