Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all OSBPL11 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by the antibody maps to a region between residue 1 to 50 of human Oxysterol Binding Protein-Like 11 using the numbering given in entry NP_073613.2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OSBPL11 Antibody

Western Blot: OSBPL11 Antibody [NBP2-36544]

Western Blot: OSBPL11 Antibody [NBP2-36544]

Western Blot: OSBPL11 Antibody [NBP2-36544] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: OSBPL11 Antibody [NBP2-36544]

Immunoprecipitation: OSBPL11 Antibody [NBP2-36544]

Immunoprecipitation: OSBPL11 Antibody [NBP2-36544] - Detection of human ORP11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP11 antibody NBP2-36544 used for IP at 6 ug per reaction. ORP11 was also immunoprecipitated by rabbit anti-ORP11 antibody NBP2-36545. For blotting immunoprecipitated ORP11, NBP2-36544 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for OSBPL11 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1: 10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: OSBPL11

OSBPL11 encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain.

Alternate Names

FLJ13012, FLJ13164, ORP11OSBP-related protein 11, ORP-11TCCCIA00292, OSBP12, oxysterol binding protein-like 11, oxysterol-binding protein-related protein 11

Gene Symbol

OSBPL11

UniProt

NP_073613.2 (Human)

Additional OSBPL11 Products

Product Documents for OSBPL11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OSBPL11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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