OSBPL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57071

Novus Biologicals, part of Bio-Techne
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NB100-57071
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all OSBPL5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KEEAFLRRRFSLC corresponding to N-Terminus according to NP_065947.1, NP_663613.1, NP_001137535.1.

Epitope

KEEAFLRRRFSLC

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Specificity

This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for OSBPL5 Antibody

Western Blot: OSBPL5 Antibody [NB100-57071]

Western Blot: OSBPL5 Antibody [NB100-57071]

Western Blot: OSBPL5 Antibody [NB100-57071] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NB100-57071]

Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NB100-57071]

Immunocytochemistry/Immunofluorescence: OSBPL5 Antibody [NB100-57071] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071] - Staining of Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for OSBPL5 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 - 0.3 ug/ml
Application Notes
Western blot: Approx. 100kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4kDa according to Human NP_065947.1 and 101.7kDa according to Rat NP_001015024.2)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OSBPL5

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified.

Alternate Names

EC 1.2.1, EC 2.7.7.6, FLJ42929, KIAA1534oxysterol-binding protein-related protein 5, OBPH1, ORP-5, ORP5oxysterol-binding protein homologue 1, OSBP-related protein 5, oxysterol binding protein-like 5, oxysterol-binding protein homolog 1, Oxysterol-binding protein homolog 1;

Entrez Gene IDs

114879 (Human); 79196 (Mouse)

Gene Symbol

OSBPL5

UniProt

NP_065947 (Human)

Additional OSBPL5 Products

Product Documents for OSBPL5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for OSBPL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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