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OSBPL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81403

Novus Biologicals, part of Bio-Techne
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NBP1-81403
NBP1-81403-25ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FSLCPPSSTPQKVDPRKLTRNLLLSGDNELYPLSPGKDMEPNGPSLPRDEGPPTPSSATKVPPAEYRLCNGSDKECVSPTARVTKKETLK

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OSBPL5 Antibody

Western Blot: OSBPL5 Antibody [NBP1-81403]

Western Blot: OSBPL5 Antibody [NBP1-81403]

Western Blot: OSBPL5 Antibody [NBP1-81403] - Analysis in control (vector only transfected HEK293T lysate) and OSBPL5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NBP1-81403]

Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NBP1-81403]

Immunocytochemistry/Immunofluorescence: OSBPL5 Antibody [NBP1-81403] - Staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: OSBPL5 Antibody [NBP1-81403]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NBP1-81403]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NBP1-81403] - Staining of human liver shows low positivity in hepatocytes as expected.

Applications for OSBPL5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 3 reviews rated 5 using NBP1-81403 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OSBPL5

OSBPL5 encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified.

Alternate Names

EC 1.2.1, EC 2.7.7.6, FLJ42929, KIAA1534oxysterol-binding protein-related protein 5, OBPH1, ORP-5, ORP5oxysterol-binding protein homologue 1, OSBP-related protein 5, oxysterol binding protein-like 5, oxysterol-binding protein homolog 1, Oxysterol-binding protein homolog 1;

Gene Symbol

OSBPL5

Additional OSBPL5 Products

Product Documents for OSBPL5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OSBPL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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