OSGEP Antibody (OTI9E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00823

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI9E3

Concentration

0.82 mg/ml

View all OSGEP Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-335 of human OSGEP(NP_060277) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

36.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OSGEP Antibody (OTI9E3)

Western Blot: OSGEP Antibody (OTI9E3) [NBP2-00823]

Western Blot: OSGEP Antibody (OTI9E3) [NBP2-00823]

Western Blot: OSGEP Antibody (OTI9E3) [NBP2-00823] - Role of GON7 on KEOPS complex stability. Western blot analysis of protein expression level of the five KEOPS subunits, including LAGE3 (NBP2-32715), in lymphoblastoid cell lines from two unaffected relatives (A.II-1 and A.II-5), four individuals with the GON7 mutation p.Tyr*7, one individual with the OSGEP antibody mutations p.Arg325Gln and p.Arg280His, and one individual with GAMOS linked to WDR73 mutations. One representative western blot is shown (three independent experiments were performed). Alpha-Tubulin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722078/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: OSGEP Antibody (OTI9E3) [NBP2-00823]

Immunohistochemistry-Paraffin: OSGEP Antibody (OTI9E3) [NBP2-00823]

Immunohistochemistry-Paraffin: OSGEP Antibody (9E3) [NBP2-00823] - Staining of paraffin-embedded Human tonsil using anti-OSGEP mouse monoclonal antibody.
Flow Cytometry: OSGEP Antibody (OTI9E3) [NBP2-00823]

Flow Cytometry: OSGEP Antibody (OTI9E3) [NBP2-00823]

Flow Cytometry: OSGEP Antibody (9E3) [NBP2-00823] - Analysis of Hela cells, using anti-OSGEP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
View 7 more images

Applications for OSGEP Antibody (OTI9E3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.82 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OSGEP

O-sialoglycoprotein endopeptidases specifically cleave the polypeptide backbone of membrane glycoproteins that contain clusters of O-linked sialoglycans

Alternate Names

EC 3.4.24.57, GCPL1FLJ20411, hOSGEP, KAE1, OSGEP1, O-sialoglycoprotein endopeptidase, probable O-sialoglycoprotein endopeptidase, PRSMG1, t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP

Entrez Gene IDs

55644 (Human)

Gene Symbol

OSGEP

Additional OSGEP Products

Product Documents for OSGEP Antibody (OTI9E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OSGEP Antibody (OTI9E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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