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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot

Label

Alexa Fluor 532

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 303822

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Osteoactivin/GPNMB isoform 2
Ala22-Asn486
Accession # NP_002501

Specificity

Detects human Osteoactivin/GPNMB in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Osteoactivin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Osteoactivin/GPNMB Antibody (303822) [Alexa Fluor® 532]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Osteoactivin/GPNMB

The GPNMB gene codes for a type I transmembrane glycoprotein NBM. The GPNMB gene is present in lowly, but not highly, metastic human melanoma cell lines, therefore, it demonstrates involvement in development, proliferation, and differentiation of tissues. Isoform 1 is 572 amino acids long at a mass of nearly 64 kDA and isoform 2 exists at 560 amino acids long at approximately 62 kDA. This gene has been known to interact with various genes such as SMAD4, PLA2G4A, and ITSN2. GPNMB has been researched in its role in retinitis, lung cancer, melanoma, glioblastoma, glaucoma, and breast cancer.

Long Name

Glycoprotein Non-Metastatic Melanoma Protein B

Alternate Names

DC-HIL, GPNMB, HGFIN

Gene Symbol

GPNMB

Additional Osteoactivin/GPNMB Products

Product Documents for Osteoactivin/GPNMB Antibody (303822) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Osteoactivin/GPNMB Antibody (303822) [Alexa Fluor® 532]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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