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Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28144

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG2 Clone # glembatumumab

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

GPNMB

Clonality

Monoclonal

Host

Human

Isotype

IgG2

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG2, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'glembatumumab'.

IgG2SA is human IgG2 with S378A mutation.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA

Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA

ELISA: Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA[NBP3-28144] -

Immobilized human GPNMB his at 2 ug/mL can bind Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA , EC50=0.01720 ug/mL.

Applications for Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Osteoactivin/GPNMB

The GPNMB gene codes for a type I transmembrane glycoprotein NBM. The GPNMB gene is present in lowly, but not highly, metastic human melanoma cell lines, therefore, it demonstrates involvement in development, proliferation, and differentiation of tissues. Isoform 1 is 572 amino acids long at a mass of nearly 64 kDA and isoform 2 exists at 560 amino acids long at approximately 62 kDA. This gene has been known to interact with various genes such as SMAD4, PLA2G4A, and ITSN2. GPNMB has been researched in its role in retinitis, lung cancer, melanoma, glioblastoma, glaucoma, and breast cancer.

Long Name

Glycoprotein Non-Metastatic Melanoma Protein B

Alternate Names

DC-HIL, GPNMB, HGFIN

Gene Symbol

GPNMB

UniProt

Additional Osteoactivin/GPNMB Products

Product Documents for Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Osteoactivin/GPNMB Antibody (glembatumumab) - IgG2SA

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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