Osteocalcin Antibody (2H9F11F8)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-62801
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative)
Cited:
Rat
Applications
Validated:
ELISA, Immunohistochemistry
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 2H9F11F8
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human osteocalcin GST fusion protein
Reactivity Notes
Predicted cross-reactivities: Rabbit, Rat, Porcine, Bovine
Specificity
This product recognizes intact human and bovine osteocalcin in a "2 site" assay and reacts with osteocalcin fragments corresponding to amino acids 15-31 and 20-43 of the native molecule. It does not recognize fragments representing amino acids 1-19 or 7-19.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Osteocalcin Antibody (2H9F11F8)
Application
Recommended Usage
ELISA
1:100-1:2000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 30358084).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Osteocalcin
Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. Drugs acting primarily on bone resorption, such as bisphosphonates, induce a rapid decline in specific bone resorption markers, whereas osteocalcin levels only change after extended therapy periods, in accordance with the expected dynamics of a bone formation marker.
Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget's disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.
Long Name
Bone gamma-Carboxyglutamate [gla] Protein
Alternate Names
BGLAP, BGP, OCN
Entrez Gene IDs
632 (Human)
Gene Symbol
BGLAP
UniProt
Additional Osteocalcin Products
Product Documents for Osteocalcin Antibody (2H9F11F8)
Product Specific Notices for Osteocalcin Antibody (2H9F11F8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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