Osteopontin/OPN Antibody (001)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89443
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0003.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 001
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Osteopontin/OPN Antibody (001)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Osteopontin/OPN (Accession#: NP_001035147.1; Met 1-Asn 314).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Osteopontin/OPN Antibody (001)
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)![Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-001-Flow-Cytometry-NBP2-89443-img0001.jpg "Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443]")
Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443]
Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443] - Analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 ug,) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0002.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]")
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000)Applications for Osteopontin/OPN Antibody (001)
Application
Recommended Usage
ELISA
1:5000-1:10000
Flow Cytometry
1:25-1:100
Immunohistochemistry-Paraffin
1:1000-1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Osteopontin/OPN
Long Name
Secreted Phosphoprotein 1 [BNSP]
Alternate Names
Eta-1, OPN, Spp1
Gene Symbol
SPP1
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody (001)
Product Specific Notices for Osteopontin/OPN Antibody (001)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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