Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44052

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Product Datasheet / COA

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NBP3-07980

Alexa Fluor 350

NBP3-07980AF350

Alexa Fluor 405

NBP3-07980AF405

Alexa Fluor 488

NBP3-07980AF488

Alexa Fluor 532

NBP3-07980AF532

Alexa Fluor 594

NBP3-07980AF594

Alexa Fluor 647

NBP3-07980AF647

Alexa Fluor 700

NBP3-07980AF700

Alexa Fluor 750

NBP3-07980AF750

Allophycocyanin

NBP3-07980APC

Biotin

NBP3-07980B

CoraFluor 1

NBP3-07980CL1

DyLight 350

NBP3-07980UV

DyLight 405

NBP3-07980V

DyLight 488

NBP3-07980G

DyLight 550

NBP3-07980R

DyLight 594

NBP3-07980DL594

DyLight 650

NBP3-07980C

DyLight 680

NBP3-07980FR

DyLight 755

NBP3-07980IR

FITC

NBP3-07980F

HRP

NBP3-07980H

Janelia Fluor 525

NBP3-07980JF525

Janelia Fluor 549

NBP3-07980JF549

Janelia Fluor 585

NBP3-07980JF585

Janelia Fluor 635

NBP3-07980JF635

Janelia Fluor 646

NBP3-07980JF646

Janelia Fluor 669

NBP3-07980JF669

mFluor Violet 450 SE

NBP3-07980MFV450

mFluor Violet 500 SE

NBP3-07980MFV500

mFluor Violet 610 SE

NBP3-07980MFV610

PE

NBP3-07980PE

PerCP

NBP3-07980PCP

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ Clone # CL10686

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Osteopontin/OPN Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P10451, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

: 1:50 - 1:200

Immunohistochemistry-Paraffin

: 1:50 - 1:200

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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