Osteopontin/OPN Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1883
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Predicted:
Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DPKSKEEDKHLK corresponding to C-Terminus according to NP_001035147.1, NP_000573.1, NP_001035149.1.
Reactivity Notes
Use in Rat reported in scientific literature (PMID:35063020). Mouse reactivity reported in scientific literature (PMID:23884286).
Localization
Secreted
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Osteopontin/OPN Antibody
![Western Blot: Osteopontin/OPN Antibody [NB100-1883]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-Western-Blot-NB100-1883-img0001.jpg "Western Blot: Osteopontin/OPN Antibody [NB100-1883]")
Western Blot: Osteopontin/OPN Antibody [NB100-1883]
Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for Osteopontin/OPN Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.1-0.3 ug/ml
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35063020) WB: Approx 60kDa band observed in lysates of Human Tonsil and lysates of cell line MOLT4 and Jurkat (calculated MW of 33.8kDa according to NP_000573.1). The observed molecular weight corresponds to earlier findings in literature (Singh et al, J Biol Chem. 1995 Nov 24;270(47):28471-8; PMID: 7499354). Primary incubation was 1 hour. ICC/IF, IHC usage is reported in scientific literature (PMID: 23884286).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Osteopontin/OPN
Long Name
Secreted Phosphoprotein 1 [BNSP]
Alternate Names
Eta-1, OPN, Spp1
Gene Symbol
SPP1
UniProt
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody
Product Specific Notices for Osteopontin/OPN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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