Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33211

Novus Biologicals, part of Bio-Techne
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Alexa Fluor 350
NB100-56505AF350
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Alexa Fluor 405
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Alexa Fluor 488
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Alexa Fluor 532
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Alexa Fluor 700
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Allophycocyanin/Cy7
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DyLight 350
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DyLight 488
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DyLight 550
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DyLight 594
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DyLight 650
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DyLight 680
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DyLight 755
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Janelia Fluor 525
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Janelia Fluor 585
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Janelia Fluor 646
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mFluor Violet 450 SE
NB100-56505MFV450
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mFluor Violet 500 SE
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NB100-56505MFV610
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NB100-56505PECY55
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 98A1071

Format

Azide Free

Concentration

1.0 mg/ml

View all Osteoprotegerin/TNFRSF11B Antibodies »

Product Summary for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free

Immunogen

A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human Osteoprotegerin was used as immunogen for this antibody.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 11028444)

Specificity

The Osteoprotegerin antibody, clone 98A1071, detects all forms of Osteoprotegerin including monomeric, dimeric and Osteoprotegerin bound to RANKL (Vandooren, 2008) as well as Osteoprotegerin bound to TRAIL (Sandra, 2006).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071)Azide Free [NBP2-33211]

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071)Azide Free [NBP2-33211]

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Osteoprotegerin in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211]

Immunocytochemistry/ Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211]

Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Osteoprotegerin (98A1071) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211]

Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211]

Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Staining of human liver. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Image from the standard format of this antibody.
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Applications for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free

Application
Recommended Usage

Flow Cytometry

0.2-1 ug/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Sandra (2006))

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

reported in scientific literature (Boumans (2012))

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (Sandra (2006))

Western Blot

1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin is encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq]

Alternate Names

OCIF, TNFRSF11B

Gene Symbol

TNFRSF11B

Additional Osteoprotegerin/TNFRSF11B Products

Product Documents for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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