Otubain-1 Antibody (1C12)

Novus Biologicals, part of Bio-Techne | Catalog # H00055611-M09

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Otubain-1 Antibodies »

Product Specifications

Immunogen

OTUB1 (AAH07519, 1 a.a. ~ 271 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAEEPQQQKQEPLGSDSEGVNCLAYDEANMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK

Specificity

OTUB1 - OTU domain, ubiquitin aldehyde binding 1 (1C12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Otubain-1 Antibody (1C12)

Western Blot: Otubain-1 Antibody (1C12) [H00055611-M09]

Western Blot: Otubain-1 Antibody (1C12) [H00055611-M09]

Western Blot: Otubain-1 Antibody (1C12) [H00055611-M09] - Analysis of OTUB1 expression in Hela S3 NE (Cat # L013V3).
ELISA: Otubain-1 Antibody (1C12) [H00055611-M09]

ELISA: Otubain-1 Antibody (1C12) [H00055611-M09]

ELISA: Otubain-1 Antibody (1C12) [H00055611-M09] - Detection limit for recombinant GST tagged OTUB1 is 1 ng/ml as a capture antibody.

Applications for Otubain-1 Antibody (1C12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Otubain-1

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 1

Alternate Names

OTB1, OTU1, OTUB1, Otubain1

Entrez Gene IDs

55611 (Human)

Gene Symbol

OTUB1

UniProt

AAH07519 (Human)

Additional Otubain-1 Products

Product Documents for Otubain-1 Antibody (1C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Otubain-1 Antibody (1C12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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