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Otubain-1 Antibody (OTI5B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45879

Novus Biologicals, part of Bio-Techne
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NBP2-45879
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Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5B8

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human OTUB1(NP_060140) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

31.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Otubain-1 Antibody (OTI5B8)

Western Blot: Otubain-1 Antibody (OTI5B8) [NBP2-45879]

Western Blot: Otubain-1 Antibody (OTI5B8) [NBP2-45879]

Western Blot: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Otubain-1.
Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879]

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879]

Immunohistochemistry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of Adenocarcinoma of Human colon tissue.
Flow Cytometry: Otubain-1 Antibody (OTI5B8) [NBP2-45879]

Flow Cytometry: Otubain-1 Antibody (OTI5B8) [NBP2-45879]

Flow Cytometry: Otubain-1 Antibody (OTI5B8) [NBP2-45879] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by OTUB1 antibody.

Applications for Otubain-1 Antibody (OTI5B8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Otubain-1

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 1

Alternate Names

OTB1, OTU1, OTUB1, Otubain1

Gene Symbol

OTUB1

Additional Otubain-1 Products

Product Documents for Otubain-1 Antibody (OTI5B8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Otubain-1 Antibody (OTI5B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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