Otubain-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20957

Novus Biologicals, part of Bio-Techne
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NBP1-20957
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Otubain-1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YKEYAEDDNIYQQK corresponding to internal region according to NP_060140.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Otubain-1 Antibody

Western Blot: Otubain-1 Antibody [NBP1-20957]

Western Blot: Otubain-1 Antibody [NBP1-20957]

Western Blot: Otubain-1 Antibody [NBP1-20957] - Staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Otubain-1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 30k Da band observed in mouse brain lysates (calculated MW of 31.3 kDa according to Human NP_060140.2 and Mouse NP_598911.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Otubain-1

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 1

Alternate Names

OTB1, OTU1, OTUB1, Otubain1

Entrez Gene IDs

55611 (Human); 107260 (Mouse); 293705 (Rat)

Gene Symbol

OTUB1

UniProt

NP_060140.2 (Human)

Additional Otubain-1 Products

Product Documents for Otubain-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Otubain-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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