OTUD4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37689
![Western Blot: OTUD4 Antibody [NBP2-37689] Western Blot: OTUD4 Antibody [NBP2-37689]](https://resources.bio-techne.com/images/products/OTUD4-Antibody-Western-Blot-NBP2-37689-img0001.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody maps to a region between residue 875 to 925 of human OTU Domain Dontaining 4 using the numbering given in entry XP_005263137.1 (GeneID 54726).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for OTUD4 Antibody - BSA Free
Western Blot: OTUD4 Antibody [NBP2-37689]
Western Blot: OTUD4 Antibody [NBP2-37689] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-OTUD4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: OTUD4 Antibody [NBP2-37689]
Immunoprecipitation: OTUD4 Antibody [NBP2-37689] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-OTUD4 antibody used for IP at 6 ug per reaction. OTUD4 was also immunoprecipitated by rabbit anti-OTUD4 antibody A304-606A. For blotting immunoprecipitated OTUD4, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for OTUD4 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: OTUD4
Alternate Names
DKFZp434I0721, HIV-1 induced protein HIN-1, HIV-1-induced protein HIN-1, HSHIN1, KIAA1046HIN1DUBA6, OTU domain containing 4, OTU domain-containing protein 4
Gene Symbol
OTUD4
UniProt
Additional OTUD4 Products
Product Documents for OTUD4 Antibody - BSA Free
Product Specific Notices for OTUD4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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