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Otx2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86533

Novus Biologicals, part of Bio-Techne
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NBP1-86533
NBP1-86533-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Otx2 Antibody

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533]

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533]

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.

Applications for Otx2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 25870833).

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

Additional Otx2 Products

Product Documents for Otx2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Otx2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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