Otx2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13176
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Otx2. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Otx2 Antibody
Western Blot: Otx2 Antibody [NBP3-13176]
Western Blot: Otx2 Antibody [NBP3-13176] - Whole cell extract (37.5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otx2 antibody (NBP3-13176) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.Immunohistochemistry-Paraffin: Otx2 Antibody [NBP3-13176]
Immunohistochemistry-Paraffin: Otx2 Antibody [NBP3-13176] - Otx2 antibody detects Otx2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Otx2 stained by Otx2 antibody (NBP3-13176) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunohistochemistry-Frozen: Otx2 Antibody [NBP3-13176]
Immunohistochemistry-Frozen: Otx2 Antibody [NBP3-13176] - Otx2 antibody detects Otx2 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Otx2 stained by Otx2 antibody (NBP3-13176) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.Applications for Otx2 Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Otx2
Long Name
Orthodenticle Homolog 2
Alternate Names
homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2
Gene Symbol
OTX2
Additional Otx2 Products
Product Specific Notices for Otx2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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