OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11969

Novus Biologicals, part of Bio-Techne
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NBP2-11969SS
NBP2-11969
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0505-8S23

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all OX40 Ligand/TNFSF4 Antibodies »

Product Specifications

Immunogen

Human recombinant OX40 Ligand/TNFSF4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free

Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free [NBP2-11969]

Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free [NBP2-11969]

Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Applications for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:20-200

Western Blot

1:1000

Neutralization

50 - 200 molar excess
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.2 ml sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.

Background: OX40 Ligand/TNFSF4

CD252 is a 35 kD member of the TNF superfamily also known as OX40L and CD134L. The OX40 ligand is expressed on activated B cells and antigen presenting cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to increase proliferation and IL-2 production, and to enhance proliferation and immunoglobulin secretion in activated B cells. The RM134L antibody can inhibit OX40/OX40 ligand interactions and block the costimulatory activity of OX40L.

Alternate Names

CD252, gp34, TNFSF4

Gene Symbol

TNFSF4

UniProt

P23510

Additional OX40 Ligand/TNFSF4 Products

Product Documents for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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