OXPAT Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-60509
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine, Bovine
Cited:
Human, Mouse, Porcine
Predicted:
Sheep (94%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to a region of the human OXPAT protein, within residues 300-400. [UniProt# Q00G26]
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID: 29094660). Immunogen displays the following percentage of sequence identity for non-tested species: sheep (94%), rat (84%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for OXPAT Antibody - BSA Free
Western Blot: OXPAT AntibodyBSA Free [NB110-60509]
Western Blot: OXPAT Antibody [NB110-60509] - Detection of OXPAT in human (lane 1) and mouse (lane 2) heart lysate.Immunohistochemistry: OXPAT Antibody - BSA Free [NB110-60509]
Immunohistochemistry: OXPAT Antibody [NB110-60509] - Staining of OXPAT in paraffin embedded mouse fat.Simple Western: OXPAT AntibodyBSA Free [NB110-60509]
Simple Western: OXPAT Antibody [NB110-60509] - Simple Western lane view shows a specific band for OXPAT in 0.5 mg/ml of Human Heart (left) and Mouse Heart (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for OXPAT Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 26096040)
Immunohistochemistry
1.0-5.0 ug/ml
Immunohistochemistry-Frozen
reported in scientific literature (PMID 24648545)
Immunohistochemistry-Paraffin
1.0-5.0 ug/ml
Simple Western
10 ug/ml
Western Blot
0.5-2.0 ug/ml
Application Notes
In WB a band can be seen at approx. 50 kDa, representing the OXPAT protein.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OXPAT
Alternate Names
lipid storage droplet protein 5, LSDA5, LSDP5, MLDP, OXPAT, perilipin 5, perilipin-5
Gene Symbol
PLIN5
Additional OXPAT Products
Product Documents for OXPAT Antibody - BSA Free
Product Specific Notices for OXPAT Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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