p114RhoGEF Antibody (1972)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43546
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1972
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human p114RhoGEF. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p114RhoGEF Antibody (1972)
Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546]
Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:1000.Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546]
Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546] - Mouse thymus gland. ARHGEF18 stained by ARHGEF18 antibody [1972] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minWestern Blot: p114RhoGEF Antibody (1972) [NBP2-43546]
Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:3000Applications for p114RhoGEF Antibody (1972)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID: 30948425).
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: p114RhoGEF
Alternate Names
114 kDa Rho-specific guanine nucleotide exchange factor, EC 3.4.24, EC 3.6.1, KIAA0521rho guanine nucleotide exchange factor 18, MGC15913, p114RhoGEF, P114-RhoGEF, P114-RHO-GEF, Rho/Rac guanine nucleotide exchange factor (GEF) 18, Rho/Rac guanine nucleotide exchange factor 18, Rho-specific guanine nucleotide exchange factor p114, SA-RhoGEF, Septin-associated RhoGEF
Gene Symbol
ARHGEF18
Additional p114RhoGEF Products
Product Documents for p114RhoGEF Antibody (1972)
Product Specific Notices for p114RhoGEF Antibody (1972)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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