p120-catenin Antibody (M354)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23202
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M354
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for p120-catenin Antibody (M354)
Immunogen
Clone (M354) was generated from a peptide that includes amino acids from the central region of human p120-catenin. This peptide sequence is highly conserved in rat and mouse p120-catenin. [UniProt# O60716]
Specificity
The antibody detects a 110 kDa protein corresponding to the molecular mass of p120-catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for p120-catenin Antibody (M354)
Immunocytochemistry/Immunofluorescence: p120-catenin Antibody (M354) [NBP3-23202] -
Immunocytochemistry/Immunofluorescence: p120-catenin Antibody (M354) [NBP3-23202] - Immunocytochemical labeling of p120-catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal p120-catenin (a.a. 275-285), p120-catenin (Tyr-228), p120-catenin (Tyr-280), or p120-catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.
Western Blot: p120-catenin Antibody (M354) [NBP3-23202] -
Western Blot: p120-catenin Antibody (M354) [NBP3-23202] - Western blot analysis of p120-catenin phosphorylation in A431 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-p120-catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-p120-catenin (Thr-916) (lanes 3 & 4).Applications for p120-catenin Antibody (M354)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunoprecipitation
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: p120-catenin
Alternate Names
Cadherin-associated Src substrate, CAS, catenin (cadherin-associated protein), delta 1, catenin delta-1, CTNND, KIAA0384P120CAS, p120, p120 catenin, p120(CAS), p120(CTN), p120cas, p120ctn
Gene Symbol
CTNND1
Additional p120-catenin Products
Product Documents for p120-catenin Antibody (M354)
Product Specific Notices for p120-catenin Antibody (M354)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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