p120-catenin Antibody (PD00-43)

Western Blot: p120-catenin Antibody (PD00-43) [NBP3-32263]

Western Blot: p120-catenin Antibody (PD00-43) [NBP3-32263] - Western blot analysis of p120-catenin on SK-Br-3 cell lysates with Rabbit anti-p120-catenin antibody (NBP3-32263) at 1/1,000 dilution.

Lysates/proteins at 10 ug/Lane.

Predicted band size: 108 kDa
Observed band size: 95 kDa

Exposure time: 1 minute;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32263) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: p120-catenin Antibody (PD00-43) [NBP3-32263]

Immunohistochemistry: p120-catenin Antibody (PD00-43) [NBP3-32263] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-p120-catenin antibody (NBP3-32263) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32263) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.