p120-catenin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85383

Novus Biologicals, part of Bio-Techne
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NBP1-85383
NBP1-85383-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p120-catenin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p120-catenin Antibody

Western Blot: p120-catenin Antibody [NBP1-85383]

Western Blot: p120-catenin Antibody [NBP1-85383]

Western Blot: p120-catenin Antibody [NBP1-85383] - Analysis in human cell lines A-431 and HEK293 using Anti-CTNND1 antibody. Corresponding CTNND1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Western Blot: p120-catenin Antibody [NBP1-85383]

Western Blot: p120-catenin Antibody [NBP1-85383]

Western Blot: p120-catenin Antibody [NBP1-85383] - Analysis using Anti-CTNND1 antibody NBP1-85383 (A) shows similar pattern to independent antibody NBP2-38130 (B).
Immunocytochemistry/ Immunofluorescence: p120-catenin Antibody [NBP1-85383]

Immunocytochemistry/ Immunofluorescence: p120-catenin Antibody [NBP1-85383]

Immunocytochemistry/Immunofluorescence: p120-catenin Antibody [NBP1-85383] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
View 2 more images

Applications for p120-catenin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p120-catenin

The alpha, beta, delta, and gamma -catenins are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. alpha-catenin, a 102 kDa protein, interacts with E-cadherin associated protein, and also associates with other members of the cadherin family, such as N-cadherin and P-cadherin. The 92 kDa beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also complexes with the tumor suppressor protein APC. delta-catenin interacts with Presenilin 1 and is expressed in the brain. The gene encoding delta-catenin maps to human chromosome 5p15.2. A hemizygous loss of the gene encoding delta-catenin leads to the mental retardation associated with Cri-du-Chat syndrome. gamma-catenin, also known as plakoglobin, is an 80-88 kDa protein that binds alpha-catenin and N-cadherin. In addition, the transmembrane phosphatase PTPm associates with catenin/cadherin complexes and may regulate complex signaling.

Alternate Names

Cadherin-associated Src substrate, CAS, catenin (cadherin-associated protein), delta 1, catenin delta-1, CTNND, KIAA0384P120CAS, p120, p120 catenin, p120(CAS), p120(CTN), p120cas, p120ctn

Gene Symbol

CTNND1

Additional p120-catenin Products

Product Documents for p120-catenin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p120-catenin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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