p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73181

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all p130Cas Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

93.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free

Western Blot: p130Cas/BCAR1 Antibody (OTI1A9)Azide and BSA Free [NBP2-73181]

Western Blot: p130Cas/BCAR1 Antibody (OTI1A9)Azide and BSA Free [NBP2-73181]

Western Blot: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.
Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181]

Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181]

Immunocytochemistry/Immunofluorescence: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.
Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181]

Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181]

Immunohistochemistry: p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-73181] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody.
View 6 more images

Applications for p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p130Cas

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Gene Symbol

BCAR1

Additional p130Cas Products

Product Documents for p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p130Cas/BCAR1 Antibody (OTI1A9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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