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p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54638

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Summary for p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

Immunogen

p130 Cas (phospho Tyr410) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 410 (G-V-Y (p)-A-V) derived from Human p130 Cas .

Modification

p Tyr410

Specificity

The antibody detects endogenous levels of p130Cas/BCAR1 only when phosphorylated at tyrosine 410.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

Western Blot: p130Cas/BCAR1 [p Tyr410] Antibody [NBP2-54638] - Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas/BCAR1 [p Tyr410] Antibody. The lane on the left is treated with antigen-specific peptide.
Immunohistochemistry: p130Cas/BCAR1 [p Tyr410] Antibody [NBP2-54638] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130Cas/BCAR1 [p Tyr410] antibody (left) or the same antibody preincubated with blocking peptide (right).

Applications for p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Epitope affinity purified

Formulation

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: p130Cas

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Gene Symbol

BCAR1

Additional p130Cas Products

Product Documents for p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p130Cas/BCAR1 [p Tyr410] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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