p130Cas/BCAR1 [p Tyr165] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13075
![Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]](https://resources.bio-techne.com/images/products/p130Cas-BCAR1-[p-Tyr165]-Antibody-Western-Blot-NBP3-13075-img0003.jpg "Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr165 of human p130Cas/BCAR1. The exact sequence is proprietary.
Modification
p Tyr 165
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for p130Cas/BCAR1 [p Tyr165] Antibody
![Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]](https://resources.bio-techne.com/images/products/p130Cas-BCAR1-[p-Tyr165]-Antibody-Western-Blot-NBP3-13075-img0004.jpg "Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]")
Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]
Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]](https://resources.bio-techne.com/images/products/p130Cas-BCAR1-[p-Tyr165]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13075-img0002.jpg "Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]")
Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]
Immunocytochemistry/Immunofluorescence: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - p130Cas/BCAR1 (phospho Tyr165) antibody detects p130Cas/BCAR1 (phospho Tyr165) protein at cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: p130Cas/BCAR1 (phospho Tyr165) protein stained by p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.![Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]](https://resources.bio-techne.com/images/products/p130Cas-BCAR1-[p-Tyr165]-Antibody-Western-Blot-NBP3-13075-img0001.jpg "Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]")
Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]
Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)Applications for p130Cas/BCAR1 [p Tyr165] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: p130Cas
Long Name
Breast Cancer Anti-estrogen Resistance 1
Alternate Names
BCAR1, CASS1, CRKAS
Gene Symbol
BCAR1
Additional p130Cas Products
Product Documents for p130Cas/BCAR1 [p Tyr165] Antibody
Product Specific Notices for p130Cas/BCAR1 [p Tyr165] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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