p130Cas/BCAR1 [p Tyr762] Antibody

Biological Validation

Unconjugated

Polyclonal Rabbit IgG

Please see the vial label for concentration. If unlisted please contact technical services.

Phospho-p130Cas/BCAR1 (Tyr-762) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src-binding domain of rat p130Cas/BCAR1. This rat sequence is the same as Tyr-664 in human p130Cas/BCAR1 and Tyr-668 in mouse p130Cas/BCAR1. This site is also highly conserved in Cas-L (HEF1, Nedd9). [UniProt# Q63767]

p Tyr762

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130Cas/BCAR1 (Tyr-751) peptide before affinity purification using phospho-p130 Cas (Tyr-762) peptide (without carrier). The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells.

Polyclonal

Rabbit

IgG

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Stable for at least 1 year at -20°C.

Antigen Affinity-purified

PBS, 1 mg/ml BSA and 50% glycerol

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C.