p14ARF/CDKN2A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05521
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for p14ARF/CDKN2A Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LVTLRIRRACGPPRVRVFVVHIPRLTGEWAAPGAPAAVALVLMLLRSQRLGQQPLPRRPGHDDGQRPS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for p14ARF/CDKN2A Antibody
![Immunocytochemistry/ Immunofluorescence: p14ARF/CDKN2A Antibody [NBP3-05521]](https://resources.bio-techne.com/images/products/p14ARF-CDKN2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05521-img0001.jpg "Immunocytochemistry/ Immunofluorescence: p14ARF/CDKN2A Antibody [NBP3-05521]")
Immunocytochemistry/ Immunofluorescence: p14ARF/CDKN2A Antibody [NBP3-05521]
Immunocytochemistry/Immunofluorescence: p14ARF/CDKN2A Antibody [NBP3-05521] - Staining of human cell line PC-3 shows localization to nucleoli.Applications for p14ARF/CDKN2A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Application Notes
For ICC/IF fixation permeabilization use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: p14ARF/CDKN2A
The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded by differential splicing of alternative first exons. The p16INK4a (exon 1 alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF protein complexes with MDM2 within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
Alternate Names
ARF, CDK4 inhibitor p16-INK4, CDK4IP14ARF, CDKN2, cell cycle negative regulator beta, CMM2P16-INK4A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), INK4, INK4A, MLMP16INK4, MTS-1, MTS1P14, Multiple tumor suppressor 1, p14, p14ARF, P16, p16-INK4, P16INK4A, p16-INK4a, p19, P19ARF, TP16
Gene Symbol
CDKN2A
Additional p14ARF/CDKN2A Products
Product Documents for p14ARF/CDKN2A Antibody
Product Specific Notices for p14ARF/CDKN2A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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