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p15INK4b/CDKN2B Antibody (8C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00001030-M07

Novus Biologicals, part of Bio-Techne
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H00001030-M07

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CDKN2B (AAH14469, 1 a.a. ~ 138 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MREENKGIPSGGGSDEGLASAAARGLVEKVRQLLEAGADPNGVNRFGRRAIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEERGHRDVAGYLRTATGD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for p15INK4b/CDKN2B Antibody (8C4)

Western Blot: p15INK4b/CDKN2B Antibody (8C4) [H00001030-M07]

Western Blot: p15INK4b/CDKN2B Antibody (8C4) [H00001030-M07]

Western Blot: p15INK4b/CDKN2B Antibody (8C4) [H00001030-M07] - Analysis of CDKN2B expression in transfected 293T cell line by CDKN2B monoclonal antibody (M07), clone 8C4. Lane 1: CDKN2B transfected lysatE (14.7 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: p15INK4b/CDKN2B Antibody (8C4) [H00001030-M07] - Detection limit for recombinant GST tagged CDKN2B is 0.3 ng/ml as a capture antibody.

Applications for p15INK4b/CDKN2B Antibody (8C4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p15INK4b/CDKN2B

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]

Long Name

p15 Cyclin Dependent Kinase 4 Inhibitor 2B

Alternate Names

CDKN2B, MTS2

Gene Symbol

CDKN2B

Additional p15INK4b/CDKN2B Products

Product Documents for p15INK4b/CDKN2B Antibody (8C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p15INK4b/CDKN2B Antibody (8C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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