p15INK4b/CDKN2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26117

Novus Biologicals, part of Bio-Techne
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NBP2-26117
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all p15INK4b/CDKN2B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RQGARERGARP corresponding to C-Terminus according to NP_004927.2, NP_511042.1.

Specificity

This antibody is expected to recognize isoform 2 (NP_511042.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for p15INK4b/CDKN2B Antibody

Western Blot: p15INK4b/CDKN2B Antibody [NBP2-26117]

Western Blot: p15INK4b/CDKN2B Antibody [NBP2-26117]

Western Blot: p15INK4b/CDKN2B Antibody [NBP2-26117] - Staining of Human Gastrointestinal cancer lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for p15INK4b/CDKN2B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 12+14 kDa band bands observed in human gastrointestinal cancer lysates (calculated MW of 8.1 kDa band according to NP_511042.1). The observed 14 kDa band cannot represent isoform 1, so it most likely represents another posttranslational modification of isoform 2.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p15INK4b/CDKN2B

The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFbeta-treated human keratinocytes, suggesting that p15 may act as an effector of TGFbeta-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFbeta-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.

Long Name

p15 Cyclin Dependent Kinase 4 Inhibitor 2B

Alternate Names

CDKN2B, MTS2

Entrez Gene IDs

1030 (Human)

Gene Symbol

CDKN2B

Additional p15INK4b/CDKN2B Products

Product Documents for p15INK4b/CDKN2B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p15INK4b/CDKN2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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