p15INK4b/CDKN2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24782

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p15INK4b/CDKN2B Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein p15INK4b/CDKN2B using the following amino acid sequence: REGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p15INK4b/CDKN2B Antibody

p15INK4b/CDKN2B Antibody Immunocytochemistry/Immunofluorescence: p15INK4b/CDKN2B Antibody [NBP3-24782]

Immunocytochemistry/Immunofluorescence: p15INK4b/CDKN2B Antibody [NBP3-24782]

Staining of human cell line U2OS shows localization to nucleoplasm.

Applications for p15INK4b/CDKN2B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p15INK4b/CDKN2B

The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFbeta-treated human keratinocytes, suggesting that p15 may act as an effector of TGFbeta-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFbeta-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.

Long Name

p15 Cyclin Dependent Kinase 4 Inhibitor 2B

Alternate Names

CDKN2B, MTS2

Gene Symbol

CDKN2B

Additional p15INK4b/CDKN2B Products

Product Documents for p15INK4b/CDKN2B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for p15INK4b/CDKN2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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