p190RhoGAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80051

Novus Biologicals, part of Bio-Techne
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NBP1-80051
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all p190RhoGAP Antibodies »

Product Specifications

Immunogen

Synthetic peptide directed towards the N terminal of human GRLF1. Peptide sequence RPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHI. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

171 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for p190RhoGAP Antibody

Western Blot: p190RhoGAP Antibody [NBP1-80051]

Western Blot: p190RhoGAP Antibody [NBP1-80051]

Western Blot: p190RhoGAP Antibody [NBP1-80051] - Hela cell lysate, concentration 0.2-1 ug/ml.

Applications for p190RhoGAP Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p190RhoGAP

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq]

Alternate Names

glucocorticoid receptor DNA binding factor 1, glucocorticoid receptor DNA-binding factor 1, glucocorticoid receptor repression factor 1, GRF1, GRF-1, GRLF1, KIAA1722, MGC10745, P190A, P190-A, p190ARhoGAP, p190RhoGAP, rho GAP p190A, Rho GTPase activating protein 35, rho GTPase-activating protein 35

Gene Symbol

ARHGAP35

Additional p190RhoGAP Products

Product Documents for p190RhoGAP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p190RhoGAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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