p19ARF/CDKN2A Antibody (12-A1-1)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-169

Novus Biologicals, part of Bio-Techne
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NB200-169
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Key Product Details

Species Reactivity

Validated:

Mouse, Hamster (Negative), Human (Negative)

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 12-A1-1

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all p19ARF/CDKN2A Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a sequence (within residues 1-100) of mouse p19ARF/CDKN2A. [UniProt# Q64364]

Epitope

The epitope recognized is between amino acids 54-62.

Reactivity Notes

No reactivity with human or Golden Syrian hamster. Armenian hamster has not been tested.

Specificity

This antibody is specific for murine p19ARF/CDKN2A. It does not recognize human p14Arf nor Golden hamster p13Arf.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p19ARF/CDKN2A Antibody (12-A1-1)

Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169]

Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169]

Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).
Immunocytochemistry/ Immunofluorescence: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169]

Immunocytochemistry/ Immunofluorescence: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169]

Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with NB200-169, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).
Immunoprecipitation: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169]

Immunoprecipitation: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169]

Immunoprecipitation: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (Lanes 3), or NB200-169 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) from Arf null NIH3T3 cells (Lanes 1) and HA- p19Arf expressing cells (Lanes 2) were also analyzed by Western blotting, as input controls.

Applications for p19ARF/CDKN2A Antibody (12-A1-1)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:100

Western Blot

1:500
Application Notes
By Western blot, this antibody recognizes bands at ~19 kDa.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p19ARF/CDKN2A

ARF (alternative reading frame; p14ARF in human, p19ARF in mouse) is a tumor suppressor that serves as a sensor of hyper-proliferative signals, resulting in p53-dependent growth arrest and apoptotic cell death. ARF interacts with several proteins including COMMD1, CDKN2AIP, TBRG1, E4F1, C1QBP etc. and can bind to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI as well as UBE2I/UBC9. ARF has the capability of inducing cell cycle arrest in G1 as well as G2 phases. While ARF is not expressed at significant levels in most normal tissues, oncogene activation triggers ARF overexpression, resulting in inhibition of MDM2 ubiquitin ligase and stabilization of p53. It is induced by progesterone as well as Ras/DMTF1, and is downregulated through NFkB p65 subunit (RELA). Inhibition of the p53 pathway, most commonly via mutations in p53 itself, inactivation of ARF, or amplification of MDM2 has been proposed to be a crucial step in pathogenesis of most human malignancies. Moreover, mice with null ARF have been shown to develop tumors rapidly and to display delayed mammary gland involution.

Alternate Names

alternative reading frame, ARF, ARF-INK4a, CDK4I, CDKN2A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4), cyclin-dependent kinase inhibitor 2A, isoform 3, cyclin-dependent kinase inhibitor 2A, isoforms 1/2, cyclin-dependent kinase inhibitor protein, INK4, INK4a, Ink4a/Arf, INK4a-ARF, mitochondrial smARF', MTS1, p14, p14ARF, P16, p16(INK4a), p16-INK4, P16INK4A, p16-INK4a, P19, p19, p19Arf, Pctr1

Entrez Gene IDs

1029 (Human); 12578 (Mouse)

Gene Symbol

CDKN2A

UniProt

Q64364 (Mouse)

Additional p19ARF/CDKN2A Products

Product Documents for p19ARF/CDKN2A Antibody (12-A1-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p19ARF/CDKN2A Antibody (12-A1-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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