p21/CIP1/CDKN1A Antibody (4Z1H9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15661

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15661-20ul
NBP3-15661-100ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4Z1H9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p21/CIP1/CDKN1A Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human p21/CIP1/CDKN1A (NP_000380.1). MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p21/CIP1/CDKN1A Antibody (4Z1H9)

Western Blot: p21/CIP1/CDKN1A Antibody (4Z1H9) [NBP3-15661]

Western Blot: p21/CIP1/CDKN1A Antibody (4Z1H9) [NBP3-15661]

Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit . Exposure time: 90s.

Analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit . Exposure time: 90s.
p21/CIP1/CDKN1A Antibody (4Z1H9)

Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -

Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -analysis of lysates from wild type (WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.
View 6 more images

Applications for p21/CIP1/CDKN1A Antibody (4Z1H9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody (4Z1H9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p21/CIP1/CDKN1A Antibody (4Z1H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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