p21/CIP1/CDKN1A Antibody (CIP1/823) [Janelia Fluor® 646]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47783JF646
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative), Rat (Negative)
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 646
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # CIP1/823
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human p21/CIP1/CDKN1A protein (Uniprot: P38936)
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Nuclear
Specificity
This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Applications for p21/CIP1/CDKN1A Antibody (CIP1/823) [Janelia Fluor® 646]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: p21/CIP1/CDKN1A
Long Name
p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Alternate Names
CDKN1A, CIP1
Gene Symbol
CDKN1A
Additional p21/CIP1/CDKN1A Products
Product Documents for p21/CIP1/CDKN1A Antibody (CIP1/823) [Janelia Fluor® 646]
Product Specific Notices for p21/CIP1/CDKN1A Antibody (CIP1/823) [Janelia Fluor® 646]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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