p21/CIP1/CDKN1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1941

Novus Biologicals, part of Bio-Techne
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NB100-1941
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all p21/CIP1/CDKN1A Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the C-terminal of human p21.

Localization

Nuclear

Specificity

p21 (C19) reacts with p21(also designated WAF1/Cip1) of mouse, rat, and human origin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for p21/CIP1/CDKN1A Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:200

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.2% gelatin

Preservative

0.1% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p21/CIP1/CDKN1A

The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Entrez Gene IDs

1026 (Human); 12575 (Mouse); 114851 (Rat)

Gene Symbol

CDKN1A

UniProt

P38936

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p21/CIP1/CDKN1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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