p27/Kip1 Antibody (4B4-E6)

Novus Biologicals, part of Bio-Techne | Catalog # H00001027-M01

Novus Biologicals, part of Bio-Techne
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H00001027-M01-50ug
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B4-E6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p27/Kip1 Antibodies »

Product Specifications

Immunogen

CDKN1B (AAH01971, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATGDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

Specificity

CDKN1B - cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for p27/Kip1 Antibody (4B4-E6)

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Western Blot: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of CDKN1B expression in transfected 293T cell line by CDKN1B monoclonal antibody (M01), clone 4B4-E6.Lane 1: CDKN1B transfected lysate(22.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (4B4-E6) [H00001027-M01] - Analysis of monoclonal antibody to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 5 ug/ml.
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Applications for p27/Kip1 Antibody (4B4-E6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p27/Kip1

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Entrez Gene IDs

1027 (Human)

Gene Symbol

CDKN1B

OMIM

600778 (Human)

UniProt

AAH01971 (Human)

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (4B4-E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p27/Kip1 Antibody (4B4-E6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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