Skip to main content

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7B9

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human p27/Kip1 (1-198aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for p27/Kip1 Antibody (7B9) - BSA Free

Western Blot: p27/Kip1 Antibody (7B9)BSA Free [NBP2-59444]

Western Blot: p27/Kip1 Antibody (7B9)BSA Free [NBP2-59444]

Western Blot: p27/Kip1 Antibody (7B9) [NBP2-59444] - Lane 1: MCF-7 cell lysates, Lane 2: NIH/3T3 cell lysates
Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (7B9) - BSA Free [NBP2-59444]

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (7B9) - BSA Free [NBP2-59444]

Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (7B9) [NBP2-59444] - Analysis of CDKN1b in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDKN1b antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for p27/Kip1 Antibody (7B9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (7B9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p27/Kip1 Antibody (7B9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...