p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48401
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401]](https://resources.bio-techne.com/images/products/p27-Kip1-Antibody-DCS-72-F6-+-KIP1-769-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48401-img0001.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DCS-72.F6 + KIP1/769
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted
Immunogen
Mouse recombinant p27/Kip1 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769) (Uniprot: P46527)
Epitope
aa 83-204
Localization
Nuclear
Specificity
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401]
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401] - Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)Applications for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: p27/Kip1
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
Additional p27/Kip1 Products
Product Documents for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted
Product Specific Notices for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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