P2X4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00141

Novus Biologicals, part of Bio-Techne
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NBP1-00141
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all P2X4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YREKKYKYVEDYEQ corresponding to C-Terminus according to NP_002551.2.

Epitope

C Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for P2X4 Antibody

Western Blot: P2X4 Antibody [NBP1-00141]

Western Blot: P2X4 Antibody [NBP1-00141]

Western Blot: P2X4 Antibody [NBP1-00141] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: P2X4 Antibody [NBP1-00141]

Flow Cytometry: P2X4 Antibody [NBP1-00141]

Flow Cytometry: P2X4 Antibody [NBP1-00141] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for P2X4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: P2X4

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined. [provided by RefSeq]

Long Name

P2X Purinergic Receptor 4

Alternate Names

P2RX4

Entrez Gene IDs

5025 (Human); 18438 (Mouse); 29659 (Rat)

Gene Symbol

P2RX4

UniProt

NP_002551.2 (Human)

Additional P2X4 Products

Product Documents for P2X4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for P2X4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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