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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 545205

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for P2X7/P2RX7 Antibody (545205) [Unconjugated]

Immunogen

E. coli-derived partial recombinant human P2X7/P2RX7 protein (amino acids 356-595)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for P2X7/P2RX7 Antibody (545205) [Unconjugated]

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody (545205) [Unconjugated] [MAB97901] - P2X7/P2RX7 was detected in immersion fixed paraffin-embedded sections of human bladder using Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB97901) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membrane of epithelial cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for P2X7/P2RX7 Antibody (545205) [Unconjugated]

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P2X7/P2RX7

FUNCTION: Receptor for ATP that acts as a ligand gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.; Subcellular location: Membrane; Multi-pass membrane protein.

Long Name

P2X Purinergic Receptor 7

Alternate Names

P2RX7

Gene Symbol

P2RX7

Additional P2X7/P2RX7 Products

Product Documents for P2X7/P2RX7 Antibody (545205) [Unconjugated]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P2X7/P2RX7 Antibody (545205) [Unconjugated]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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