P2Y12/P2RY12 Antibody (PSH04-48)

Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622]

Immunohistochemistry-Frozen: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622] - Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-P2Y12/P2RY12 antibody (NBP3-32622) at 1/200 dilution.

The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32622, green) at 1/200 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622]

Immunohistochemistry: P2Y12/P2RY12 Antibody (PSH04-48) [NBP3-32622] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-P2Y12/P2RY12 antibody (NBP3-32622) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32622) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.