p38 alpha [p Tyr182] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-82097
![Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]](https://resources.bio-techne.com/images/products/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0001.jpg "Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]")
![Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]](https://resources.bio-techne.com/images/products/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0005.jpg "Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p38 alpha around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).
Modification
p Tyr182
Specificity
Detects endogenous levels of p38 alpha only when phosphorylated at tyrosine 182.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p38 alpha [p Tyr182] Antibody
![Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]](https://resources.bio-techne.com/images/products/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0008.jpg "Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]")
Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097]
Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.
Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] -
Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82097 (left) or the same antibody preincubated with blocking peptide(right).Applications for p38 alpha [p Tyr182] Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:300
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:500-1:2000
Reviewed Applications
Read 1 review rated 4 using NB100-82097 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 50% glycerol, 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: p38 alpha
Alternate Names
MAPK14, SAPK2a
Gene Symbol
MAPK14
UniProt
Additional p38 alpha Products
Product Documents for p38 alpha [p Tyr182] Antibody
Product Specific Notices for p38 alpha [p Tyr182] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...